8BM3
H207A mutant of E. coli PgpB, a PAP2 type phosphatidyl glycerol phosphate and C55-PP phosphatase, in complex with farnesyl pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978570 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 88.720, 61.620, 144.240 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 47.770 - 3.500 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jwy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 3.710 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 19616 | 3084 |
| <I/σ(I)> | 4.82 | 1.02 |
| Completeness [%] | 97.6 | 95.8 |
| Redundancy | 2.5 | 2.51 |
| CC(1/2) | 0.990 | 0.502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCl pH 7, ammonium sulfate 2,77 M |
