8BLZ

Engineered Fructosyl Peptide Oxidase - D02 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 21
Unit cell lengths	91.096, 129.493, 86.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.750 - 1.700
R-factor	0.16896
Rwork	0.168
R-free	0.19353
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y4j
RMSD bond length	0.012
RMSD bond angle	1.723
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.750	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	55830	2946
<I/σ(I)>	12.6	2.2
Completeness [%]	98.7
Redundancy	12.7
CC(1/2)	1.000	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 20000 16-26%, MES 0.1M pH 6.5