8BK4

Full length structure of the apo-state LpMIP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	42.493, 57.529, 67.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.830 - 1.340
Rwork	0.197
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jvw
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.840	1.390
High resolution limit [Å]	1.340	1.342
Rmeas	0.080	0.100
Number of reflections	28364	209
<I/σ(I)>	15.56
Completeness [%]	74.8
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	30 %(v/v) MPD, 0.2 M ammonium acetate, 0.1 M sodium citrate, pH 5.6