8BJW

Structure of Tupaia Paramyxovirus C protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.07197
Spacegroup name	P 32 2 1
Unit cell lengths	136.734, 136.734, 63.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.460 - 2.390
R-factor	0.2205
Rwork	0.219
R-free	0.25420
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.585
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.460	67.800
High resolution limit [Å]	2.390	2.590
Rmerge	0.085	0.100
Number of reflections	27136	24104
<I/σ(I)>	19
Completeness [%]	100.0	100
Redundancy	17.8	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	PEG 3350, ammonium citrate dibasic
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	PEG 3350, ammonium citrate dibasic