8BJR

Crystal structure of Paradendryphiella salina PL7C alginate lyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.6888
Spacegroup name	P 1
Unit cell lengths	33.791, 39.804, 42.527
Unit cell angles	73.63, 76.35, 70.07

Refinement procedure

Resolution	27.500 - 1.100
R-factor	0.1277
Rwork	0.127
R-free	0.14990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ywf
RMSD bond length	0.006
RMSD bond angle	0.922
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.500	1.140
High resolution limit [Å]	1.100	1.100
Number of reflections	72994	7116
<I/σ(I)>	7.41
Completeness [%]	90.1
Redundancy	3.8
CC(1/2)	1.000	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M (NH4)2SO4; 25% PEG4000