8BIZ
Cystathionine gamma-lyase from Toxoplasma gondii in complex with cysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 158.346, 158.346, 94.213 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.412 - 1.891 |
| R-factor | 0.1713 |
| Rwork | 0.171 |
| R-free | 0.18610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nmp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.919 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.412 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 108481 | 5370 |
| <I/σ(I)> | 28.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20 | |
| CC(1/2) | 0.999 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 12% (w/v) PEG 3350, 0.1M sodium citrate pH 4.6, CYMAL-3 |
