8BIV

Cystathionine gamma-lyase N360S mutant from Toxoplasma gondii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 3 2 1
Unit cell lengths	157.738, 157.738, 93.331
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	93.331 - 2.220
R-factor	0.19558
Rwork	0.195
R-free	0.21605
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nmp
RMSD bond length	0.002
RMSD bond angle	1.189
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.331	2.260
High resolution limit [Å]	2.220	2.220
Number of reflections	65911	3250
<I/σ(I)>	12.9
Completeness [%]	100.0
Redundancy	30
CC(1/2)	0.997	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	12% (w/v) PEG 3350 0.1M sodium citrate pH 4.6 CYMAL-4