Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2015-11-26 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 200.769, 131.641, 119.168 |
| Unit cell angles | 90.00, 100.19, 90.00 |
Refinement procedure
| Resolution | 47.560 - 2.560 |
| R-factor | 0.2547 |
| Rwork | 0.254 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cof |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.491 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.560 | 2.880 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 42005 | 235 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 92.2 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.993 | 0.304 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 279 | 19 % PEG 1000 0.1 M sodium chloride 0.15 M ammonium sulfate 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.5 25 mM Sucrose monodecanoate detergent (sucrose monocaprate) |
