8BF4

Crystal structure of Mouse Plexin-B1 (20-535) in complex with VHH15 and VHH14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873127
Spacegroup name	P 1 21 1
Unit cell lengths	95.267, 109.233, 95.478
Unit cell angles	90.00, 90.70, 90.00

Refinement procedure

Resolution	95.260 - 2.150
R-factor	0.2118
Rwork	0.211
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8bb7
RMSD bond length	0.005
RMSD bond angle	0.801
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.653	95.470	2.540
High resolution limit [Å]	2.149	11.890	2.480
Rmerge	0.088	0.045	0.679
Rmeas	0.121	0.063	0.938
Rpim	0.084	0.045	0.645
Number of reflections	76707	663	4482
<I/σ(I)>	8.3
Completeness [%]	99.9
Redundancy	3.4	3	3.4
CC(1/2)	0.984	0.925	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	15% (w/v) PEG 6000, 0.1 M sodium citrate, pH 5.5, 3% dextran sulphate (Mr 5000)