8BD9

Crystal structure of TRIM33 alpha PHD-Bromo domain in complex with 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 65 2 2
Unit cell lengths	79.953, 79.953, 134.945
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.680 - 3.200
R-factor	0.23895
Rwork	0.232
R-free	0.34854
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u5m
RMSD bond length	0.005
RMSD bond angle	1.413
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.950	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.066	0.545
Rmeas	0.077	0.625
Rpim	0.037	0.294
Number of reflections	4598	633
<I/σ(I)>	11.2	2.3
Completeness [%]	99.5	99.8
Redundancy	3.8	4.1
CC(1/2)	0.999	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	0.2 M calcium chloride and 20 % wt/vol PEG-3350