8BCH
Human Brr2 Helicase Region in complex with Sulfaguanidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.290, 149.530, 141.490 |
| Unit cell angles | 90.00, 120.32, 90.00 |
Refinement procedure
| Resolution | 47.200 - 2.870 |
| R-factor | 0.2835 |
| Rwork | 0.281 |
| R-free | 0.33740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f91 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.040 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmeas | 0.201 | |
| Number of reflections | 58705 | 9524 |
| <I/σ(I)> | 10.19 | 0.63 |
| Completeness [%] | 97.1 | 98.4 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.998 | 0.380 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 0.1M sodium citrate, 1.5M sodium malonate |
