8BCE

Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 76

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	100.054, 119.049, 188.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.510 - 2.050
R-factor	0.2211
Rwork	0.220
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s8q
RMSD bond length	0.009
RMSD bond angle	1.006
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.050	2.050
Rmeas	0.099
Number of reflections	139806	21677
<I/σ(I)>	13.63	1.03
Completeness [%]	99.2	96.2
Redundancy	6.5	5.6
CC(1/2)	0.999	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350