8BCB
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 34
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.205, 119.231, 186.728 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.390 - 2.380 |
R-factor | 0.2061 |
Rwork | 0.205 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6s8q |
RMSD bond length | 0.010 |
RMSD bond angle | 1.075 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmeas | 0.183 | 0.183 |
Number of reflections | 89385 | 13922 |
<I/σ(I)> | 9.2 | 0.75 |
Completeness [%] | 98.7 | 96.4 |
Redundancy | 6.7 | |
CC(1/2) | 0.998 | 0.242 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350 |