8BC8
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.600, 119.061, 187.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.330 - 2.390 |
R-factor | 0.2076 |
Rwork | 0.207 |
R-free | 0.24470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6s8q |
RMSD bond length | 0.009 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmeas | 0.169 | 0.169 |
Number of reflections | 89613 | 14187 |
<I/σ(I)> | 9.57 | 0.81 |
Completeness [%] | 99.7 | 98.9 |
Redundancy | 6.7 | |
CC(1/2) | 0.998 | 0.289 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | 0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350 |