8BC7
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex an aminoglutarimide degron peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-23 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.606, 59.450, 89.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.446 - 1.719 |
| Rwork | 0.174 |
| R-free | 0.21110 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.760 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.446 | 1.820 |
| High resolution limit [Å] | 1.719 | 1.720 |
| Rmerge | 0.104 | 1.910 |
| Number of reflections | 32675 | 5210 |
| <I/σ(I)> | 12.2 | 1.18 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.05 | 13.54 |
| CC(1/2) | 0.999 | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.5 M (NH4)H2PO4 |
