8BBR
Determination of the structure of active tyrosinase from bacterium Verrucomicrobium spinosum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-26 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.298, 63.443, 117.311 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 116.370 - 1.640 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z11 4z12 5ce9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.586 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.370 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 75699 | 3073 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 98.2 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v PEG5000MME |
