8BB6
Crystal structure of Arabidopsis thaliana sucrose transporter SUC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 58.776, 65.622, 82.813 |
Unit cell angles | 89.96, 101.74, 94.72 |
Refinement procedure
Resolution | 34.740 - 2.680 |
R-factor | 0.2711 |
Rwork | 0.270 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | RoseTTAFold predicted model |
Data reduction software | DIALS (3.3.2) |
Data scaling software | xia2 (3.3.2) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.350 | 57.360 | 2.730 |
High resolution limit [Å] | 2.680 | 7.270 | 2.680 |
Rmeas | 0.314 | 0.085 | 1.985 |
Rpim | 0.171 | 0.047 | 1.073 |
Total number of observations | 110948 | 5412 | 5385 |
Number of reflections | 33404 | 1697 | 1586 |
<I/σ(I)> | 4.7 | 19.5 | 0.8 |
Completeness [%] | 98.6 | 99.7 | 95.1 |
Redundancy | 3.3 | 3.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.3 | 292 | 43.5% PEG 400, 0.25 M Ammonium dihydrogen phosphate, 0.15 M BIS-TRIS propane |