8BAP
Eugenol Oxidase (EUGO) from Rhodococcus jostii RHA1, eightfold mutant active on propanol syringol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000002 |
Spacegroup name | P 1 |
Unit cell lengths | 113.556, 142.995, 154.600 |
Unit cell angles | 114.87, 97.00, 93.28 |
Refinement procedure
Resolution | 49.044 - 2.300 |
R-factor | 0.1956 |
Rwork | 0.193 |
R-free | 0.24940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fxd |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3546) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.137 | 0.707 |
Number of reflections | 379398 | 18712 |
<I/σ(I)> | 4.6 | 1.1 |
Completeness [%] | 98.2 | 97.6 |
Redundancy | 3.5 | |
CC(1/2) | 0.987 | 0.537 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | Morpheus screening, condition A6 |