8BAO
Dysgonamonadaceae bacterium CRISPR ancillary nuclease 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.133, 109.429, 63.043 |
| Unit cell angles | 90.00, 101.88, 90.00 |
Refinement procedure
| Resolution | 54.710 - 2.060 |
| R-factor | 0.2216 |
| Rwork | 0.220 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.954 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.715 | 2.093 |
| High resolution limit [Å] | 2.058 | 2.058 |
| Rmerge | 0.240 | 2.405 |
| Rmeas | 0.260 | 2.593 |
| Rpim | 0.100 | 0.963 |
| Number of reflections | 63804 | 3196 |
| <I/σ(I)> | 5.1 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | |
| CC(1/2) | 0.993 | 0.342 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 287 | 0.1M Imidazole; MES monohydrate (acid) 0.03M Sodium fluoride; 0.03M Sodium bromide; 0.03M Sodium iodide 20% v/v Glycerol; 10% w/v PEG 4000 208.3uM cA4 |
