8B9T

Crystal structure of Scribble PDZ1 with human papillomavirus strain 16 E6 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 61 2 2
Unit cell lengths	70.611, 70.611, 140.782
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.230 - 2.500
R-factor	0.2747
Rwork	0.274
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xa6
RMSD bond length	0.015
RMSD bond angle	1.612
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.230	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.101	1.363
Number of reflections	7707	732
<I/σ(I)>	23.29	1.61
Completeness [%]	99.4	97.98
Redundancy	6.2
CC(1/2)	0.999	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	2.5 M NaCl, 0.1 m Na HEPES pH 8.0