8B8I

Nanobody (NbLumSyt1) bound to human Syt1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-29
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	87.955, 100.226, 103.939
Unit cell angles	90.00, 100.83, 90.00

Refinement procedure

Resolution	59.110 - 2.750
Rwork	0.263
R-free	0.29770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i2g
RMSD bond length	0.007
RMSD bond angle	1.185
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.570	2.850
High resolution limit [Å]	2.750	2.750
Number of reflections	46246	4523
<I/σ(I)>	10.3
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.998	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	1.6 M DL-malic acid