8B8F
Atomic structure of the beta-trefoil domain of the Laccaria bicolor lectin LBL in complex with lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 52.530, 61.270, 44.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.200 - 1.000 |
R-factor | 0.1408 |
Rwork | 0.140 |
R-free | 0.15230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y9f |
RMSD bond length | 0.008 |
RMSD bond angle | 1.269 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.200 | 1.050 |
High resolution limit [Å] | 1.000 | 1.000 |
Rpim | 0.044 | 0.044 |
Number of reflections | 79659 | 11493 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 100.0 | |
Redundancy | 8 | |
CC(1/2) | 0.988 | 0.988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (w/v) PEG 6000, 0.1 M sodium acetate, pH 5.0, and 0.2 M ammonium chloride |