8B77
The crystal structure of N828V variant of DNA Pol Epsilon containing dATP in the polymerase active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.994, 71.030, 155.255 |
| Unit cell angles | 90.00, 112.99, 90.00 |
Refinement procedure
| Resolution | 65.490 - 2.700 |
| R-factor | 0.2381 |
| Rwork | 0.236 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m8o |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.800 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.140 | 0.790 |
| Number of reflections | 43465 | 4612 |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 98.6 | 99.7 |
| Redundancy | 4.2 | |
| CC(1/2) | 0.993 | 0.420 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 10mM Tris, 15% PEG8K,10mM CaCl2 |
