8B73

Acetivibrio clariflavus beta-1,4-xylanase of Glycoside Hydrolase Family 10 (AcXyn10A).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	80.566, 88.916, 173.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.089 - 2.240
Rwork	0.206
R-free	0.23160
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.950
Data reduction software	xia2
Data scaling software	xia2.multiplex
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.580	2.290
High resolution limit [Å]	2.240	2.240
Rmerge	0.166	2.685
Rmeas	0.173
Rpim	0.050	0.764
Number of reflections	61326	3029
<I/σ(I)>	8
Completeness [%]	99.8	100
Redundancy	12.7	13.5
CC(1/2)	0.997	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	293.15	H6 conditions of JCSG+ HTS 0.1 M Ammonium acetate 0.1 M BIS-Tris, 17 % w/v PEG 10,000 109.79mM Xyltetraose (10X) with AcXynA protein in buffer containing 50mM NaCl, 20mM Tris-Cl