8B6U
Mpf2Ba1 monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-06-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 127.600, 127.600, 116.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.130 - 2.134 |
| Rwork | 0.209 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qp2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.860 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.250 | 2.250 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.104 | 0.707 |
| Number of reflections | 53473 | 7740 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M MgCl2 hexahydrate 0.1M HEPES pH=7.5 30% PEG 400 |
