8B6E

crystal structure of the DNA-binding short chromatophore-targeted protein sCTP-23166 from Paulinella chromatophora

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9200
Spacegroup name	P 1
Unit cell lengths	28.887, 30.943, 31.188
Unit cell angles	83.99, 63.45, 69.84

Refinement procedure

Resolution	29.000 - 1.200
R-factor	0.1333
Rwork	0.131
R-free	0.17630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alphafold
RMSD bond length	0.015
RMSD bond angle	1.993
Data reduction software	AutoProcess
Data scaling software	AutoProcess
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	1.243
High resolution limit [Å]	1.200	1.200
Rmerge	0.070
Number of reflections	27013	2610
<I/σ(I)>	12.04
Completeness [%]	93.0
Redundancy	3.6
CC(1/2)	0.997	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.1 M sodium HEPES pH 7.5, 15 % (w/v) PEG 20000