8B5S
Crystal Structure of P. aeruginosa WaaG in complex with UDP-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.633, 94.426, 70.170 |
| Unit cell angles | 90.00, 101.41, 90.00 |
Refinement procedure
| Resolution | 57.540 - 1.950 |
| R-factor | 0.18173 |
| Rwork | 0.180 |
| R-free | 0.21905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iv7 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.870 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.551 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 182700 | 3796 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.979 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.06M Divalents, 0.1M Buffer System 3 pH 8.5, 30.0% v/v P500MME_P20K |
