8B4K

Rfa1-N-terminal domain in complex with phosphorylated Ddc2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	54.506, 34.600, 99.350
Unit cell angles	90.00, 90.05, 90.00

Refinement procedure

Resolution	54.510 - 1.550
R-factor	0.1692
Rwork	0.168
R-free	0.19030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5omb
RMSD bond length	0.011
RMSD bond angle	1.361
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.510	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.039	0.292
Number of reflections	54483	5391
<I/σ(I)>	19.8
Completeness [%]	99.6	98.9
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	0.2M Lithium chloride, 0.1M Sodium acetate, pH 5.0, 20% w/v PEG 6000