8B4C
ToxR bacterial transcriptional regulator bound to 20 bp toxT promoter DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.500, 54.209, 63.657 |
| Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
| Resolution | 63.590 - 2.070 |
| R-factor | 0.18494 |
| Rwork | 0.182 |
| R-free | 0.24107 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PhoB DNA binding domain (1GXP) |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.729 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.590 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.105 | 1.016 |
| Number of reflections | 14625 | 1451 |
| <I/σ(I)> | 16.32 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG 4000, 0.1 M sodium acetate pH 4, 0.15 M ammonium acetate |
