8B46
Crystal structure of the SUN1-KASH6 9:9 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.6702 |
Spacegroup name | P 63 |
Unit cell lengths | 134.094, 134.094, 106.586 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.890 - 1.670 |
R-factor | 0.1669 |
Rwork | 0.166 |
R-free | 0.18960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6r2i |
RMSD bond length | 0.014 |
RMSD bond angle | 1.296 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 116.129 | 1.889 |
High resolution limit [Å] | 1.665 | 1.665 |
Rmerge | 0.133 | 1.887 |
Rpim | 0.043 | 0.622 |
Number of reflections | 70755 | 3538 |
<I/σ(I)> | 17.4 | 1.9 |
Completeness [%] | 55.7 | 8.9 |
Redundancy | 21.1 | |
CC(1/2) | 0.999 | 0.676 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M Magnesium chloride, 0.1 M MES pH 6.0, 3% w/v PEG 6000 (+25% ethylene glycol cryoprotectant) |