8B3K

Crystal structure of human Plexin-B1 (20-535) in the unbound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	I 41 2 2
Unit cell lengths	266.692, 266.692, 108.728
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.670 - 2.685
Rwork	0.199
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ol2
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	188.580	188.580	2.890
High resolution limit [Å]	2.685	11.200	2.800
Rmerge	0.374	0.063	4.090
Rmeas	0.403	0.068	4.409
Rpim	0.152	0.026	1.640
Number of reflections	48266	862	4382
<I/σ(I)>	5.9
Completeness [%]	100.0
Redundancy	13.5	11.6	14
CC(1/2)	0.993	0.998	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	1.0 M sodium acetate, 0.2 M cadmium sulphate, 0.1 M HEPES, pH 7.5