8B2R
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikF with a rice (Oryza sativa) RGA5 HMA domain mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.906, 57.896, 76.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.242 - 1.220 |
| Rwork | 0.150 |
| R-free | 0.20370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7b1i |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.808 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.242 | 1.240 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.069 | 1.719 |
| Number of reflections | 42985 | 1915 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.4 | |
| Redundancy | 11.5 | |
| CC(1/2) | 1.000 | 0.324 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 0.1 M BIS-TRIS pH 5.5 25% PEG3350 |
