8AY2

Crystal structure of the C-terminal part of rat Sec8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97944
Spacegroup name	I 4 2 2
Unit cell lengths	190.004, 190.004, 175.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.500
R-factor	0.1904
Rwork	0.189
R-free	0.21490
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.990	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.134
Rmeas	0.138
Rpim	0.035
Number of reflections	94874	2815
<I/σ(I)>	20.47
Completeness [%]	89.2
Redundancy	14.9
CC(1/2)	0.999	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	297	0.1M 2-(N-morpholino)ethanesulfonic acid, 1.5-2M NaCl, 10-15% (v/v) PEG6000, 2mM MgCl2, 10mM DTT