8AXY
Staphylococcus aureus endonuclease IV orthorhombic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.857, 82.581, 105.091 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.545 - 1.050 |
| Rwork | 0.136 |
| R-free | 0.15770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xp3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.566 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.545 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.063 | 1.882 |
| Number of reflections | 156665 | 7372 |
| <I/σ(I)> | 14.2 | 0.5 |
| Completeness [%] | 99.1 | 95.8 |
| Redundancy | 7.2 | 6.1 |
| CC(1/2) | 1.000 | 0.319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | - 0.1 M HEPES pH 7.0 - 2.2 M Ammonium sulfate |
