8AXW

The structure of mouse AsterC (GramD1c) with Ezetimibe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 41 21 2
Unit cell lengths	49.625, 49.625, 144.775
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.940 - 1.600
R-factor	0.1724
Rwork	0.172
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gqf
RMSD bond length	0.013
RMSD bond angle	1.188
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.290	0.529
Number of reflections	24703	2330
<I/σ(I)>	30.34	2.52
Completeness [%]	99.6
Redundancy	22.4
CC(1/2)	0.976	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M sodium chloride, 0.1 M MES pH 6.5, 10% PEG 4000