8AXT

Sialidases and Fucosidases of Akkermansia muciniphila are key for rapid growth on colonic mucin and nutrient sharing amongst mucin-associated human gut microbiota

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	72.960, 56.790, 146.720
Unit cell angles	90.00, 94.55, 90.00

Refinement procedure

Resolution	72.730 - 1.590
R-factor	0.17
Rwork	0.168
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alpha fold model
RMSD bond length	0.009
RMSD bond angle	1.051
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	146.258	1.630
High resolution limit [Å]	1.590	1.590
Rmerge	0.300	1.000
Rmeas	0.320	1.000
Rpim		1.000
Number of reflections	160846	11798
<I/σ(I)>	6.7
Completeness [%]	99.8	99.8
Redundancy	6.7	6.6
CC(1/2)	0.990	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	25% PEG 3350, 0.1 M Bis tris, 0.2 MgCl2