8AXC

Crystal structure of mouse Ces2c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.012100
Spacegroup name	P 21 21 21
Unit cell lengths	98.052, 143.595, 183.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.050 - 2.120
Rwork	0.163
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mx9
RMSD bond length	0.010
RMSD bond angle	1.534
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	2.196
High resolution limit [Å]	2.120	2.120
Rmerge	0.091	0.533
Number of reflections	145761	14158
<I/σ(I)>	15.23
Completeness [%]	99.0	97.4
Redundancy	6.9	6.3
CC(1/2)	0.998	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Crystallisation condition 0.5 ul with PEG 8000, 0.2 M Ammonium sulfate 0.1 M Sodium cacodylate, pH-6.5, 30 % w/v and 0.5 ul 10mg/ml Protein in 150mM NaCl, 20mM Tris HCl