8AX9

Human Apolipoprotein E4 (ApoE4) N-terminal domain (space group P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	45.593, 53.175, 73.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.805 - 1.549
R-factor	0.1848
Rwork	0.184
R-free	0.19510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gs9
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.590	45.590	1.580
High resolution limit [Å]	1.549	8.480	1.550
Rmerge	0.065	0.019	1.800
Rmeas	0.067	0.020	1.966
Rpim	0.019	0.006	0.555
Number of reflections	26880	209	1324
<I/σ(I)>	23.1
Completeness [%]	100.0	99.7	99.8
Redundancy	13	10.1	12.2
CC(1/2)	0.999	0.999	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	HEPES, PEG3350