8AX4

Crystal structure of FMV N in its RNA-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072
Spacegroup name	P 1 21 1
Unit cell lengths	99.870, 96.920, 103.520
Unit cell angles	90.00, 111.92, 90.00

Refinement procedure

Resolution	48.460 - 2.280
R-factor	0.2168
Rwork	0.217
R-free	0.22040
Structure solution method	SIRAS
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.460	48.460	2.340
High resolution limit [Å]	2.280	10.200	2.280
Rmerge	0.067	0.012	1.618
Rmeas	0.079	0.014	1.897
Total number of observations	296115
Number of reflections	82002	953	6021
<I/σ(I)>	15.31	76.99	0.94
Completeness [%]	98.8	96.1	99.2
Redundancy	3.611	3.687	3.643
CC(1/2)	0.999	1.000	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	3.45 mM Sodium Formate