8AWI

Crystal structure of Human Transthyretin at 1.15 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97993
Spacegroup name	P 21 2 21
Unit cell lengths	42.853, 64.325, 85.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.325 - 1.150
R-factor	0.14846
Rwork	0.147
R-free	0.16580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in-house model
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.325	1.170
High resolution limit [Å]	1.150	1.150
Number of reflections	84053	39058
<I/σ(I)>	19.5
Completeness [%]	98.8
Redundancy	23.1
CC(1/2)	0.996	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	protein at 5.2mg/ml in 10mM sodium phosphate pH7.6 and 100mM potassium chloride was mixed 1:1 with 1.3-1.6M sodium citrate and 3.5% glycerol