8AVJ

Structure of short apo agroavidin with the Hoef tail.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.965
Spacegroup name	P 32 2 1
Unit cell lengths	116.697, 116.697, 147.889
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.300 - 2.100
R-factor	0.20398
Rwork	0.203
R-free	0.23029
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8asu
RMSD bond length	0.014
RMSD bond angle	1.778
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.134
High resolution limit [Å]	2.100	2.100
Rmerge	0.051	0.613
Number of reflections	64372	16884
<I/σ(I)>	0.998
Completeness [%]	99.3
Redundancy	5.2
CC(1/2)	0.998	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% ethylene glycol 0.05M acetic acid pH 3.2