8AU6
Structure of Corynebacterium glutamicum Cg1604, a cell division regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-28 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979261 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.780, 105.500, 70.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.040 - 2.000 |
R-factor | 0.1957 |
Rwork | 0.194 |
R-free | 0.22850 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXD |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.100 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rpim | 0.035 | 0.642 |
Number of reflections | 17209 | 1207 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 99.7 | 95.9 |
Redundancy | 14.6 | 11.5 |
CC(1/2) | 0.999 | 0.551 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 30 % (w/v) PEG 4K, 0.2 M MgCl2, 0.1 M TRIS pH 8.5 |