8ASU
The Crystal structure of short F46Y agroavidin - biotin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-23 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.365, 81.973, 68.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.090 - 2.520 |
| R-factor | 0.20762 |
| Rwork | 0.204 |
| R-free | 0.27309 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ass |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.727 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.750 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Number of reflections | 15697 | 1118 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 98.5 | 98.5 |
| Redundancy | 4.6 | 4.7 |
| CC(1/2) | 0.998 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.1 M Bis Tris |
