8ASK

Crystal structure of d(GCCCACCACGGC)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	270
Detector technology	PIXEL
Collection date	2022-08-03
Detector	DECTRIS PILATUS 2M
Wavelength(s)	2.00
Spacegroup name	H 3
Unit cell lengths	64.490, 64.490, 46.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.590 - 2.955
Rwork	0.125
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	X3DNA
RMSD bond length	0.006
RMSD bond angle	1.196
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.590	3.020
High resolution limit [Å]	2.950	2.970
Number of reflections	2783	1399
<I/σ(I)>	2
Completeness [%]	97.0
Redundancy	9.2
CC(1/2)	0.992	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	20%MPD, 120mMMgCl2, 60mM NaCaCo, 2mM Spermine