8AS4
Crystal structure of human beta-arrestin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.990, 71.390, 115.370 |
| Unit cell angles | 90.00, 97.77, 90.00 |
Refinement procedure
| Resolution | 44.980 - 2.300 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g4m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.980 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.240 | 2.460 |
| Rpim | 0.070 | 0.780 |
| Number of reflections | 44486 | 4409 |
| <I/σ(I)> | 12.1 | 1.6 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 27.1 | 27 |
| CC(1/2) | 0.990 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.02 M Calcium chloride 0.02 M Cadmium chloride hydrate, 0.02 M Cobalt(II) chloride hexahydrate, 20%PEG3350 |
