8AS3
Structure of arrestin2 in complex with 6P CCR5 phosphopeptide and Fab30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999998 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 116.314, 121.064, 145.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.310 - 3.500 |
| R-factor | 0.2869 |
| Rwork | 0.284 |
| R-free | 0.33260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jqi |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.310 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.144 | 4.240 |
| Rpim | 0.040 | 1.465 |
| Number of reflections | 13152 | 1295 |
| <I/σ(I)> | 13.2 | 0.7 |
| Completeness [%] | 98.2 | 90.3 |
| Redundancy | 13.6 | 13.6 |
| CC(1/2) | 0.999 | 0.259 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1% tryptone (w/v), 0.05M HEPES, 12 % PEG3,350 |
