8ARK

Crystal structure of DEAD-box protein Dbp2 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977770
Spacegroup name	C 1 2 1
Unit cell lengths	204.103, 115.124, 81.411
Unit cell angles	90.00, 101.92, 90.00

Refinement procedure

Resolution	38.850 - 3.220
R-factor	0.2251
Rwork	0.224
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i4i
RMSD bond length	0.005
RMSD bond angle	1.033
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.850	3.330
High resolution limit [Å]	3.220	3.220
Rmerge	0.063	0.578
Number of reflections	28601	2971
<I/σ(I)>	15.1	2.63
Completeness [%]	94.5	98.5
Redundancy	3.4	3.4
CC(1/2)	0.998	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.12 M Monosaccharides 1 M Sodium HEPES; MOPS (acid) pH 7.5 19% w/v PEG 500MME PEG 20000