8AR8

Bovine glutamate dehydrogenase in complex with ADP at 2.4 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 1
Unit cell lengths	87.510, 92.030, 119.570
Unit cell angles	99.35, 106.73, 109.73

Refinement procedure

Resolution	47.460 - 2.400
R-factor	0.2052
Rwork	0.204
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jcz
RMSD bond length	0.006
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.458	2.484
High resolution limit [Å]	2.400	2.400
Rpim	0.175	0.398
Number of reflections	96056	4805
<I/σ(I)>	5	2
Completeness [%]	89.9	86
Redundancy	3.4	3.6
CC(1/2)	0.925	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20 mM CaCl2, 100 mM Na-acetate buffer (pH 4.6), 30% MPD (2-methyl-2,4-pentanediol)