8AQ4
In surfo structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli in complex with TITC and lyso-PE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96862 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 160.111, 160.111, 90.934 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.130 - 2.620 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n6h |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3600) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.130 | 3.010 |
High resolution limit [Å] | 2.620 | 2.660 |
Rmeas | 0.260 | 1.550 |
Number of reflections | 24528 | 1226 |
<I/σ(I)> | 8 | 1.7 |
Completeness [%] | 93.2 | 60.8 |
Redundancy | 9.8 | 9.2 |
CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 50 mM sodium acetate pH 5.0, 50 mM magnesium acetate and 28-36 %(v/v) PEG200 |