8AP3

Structure of A. thaliana MIF/D-DT-like protein-2 (MDL2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999
Spacegroup name	P 21 21 21
Unit cell lengths	46.218, 79.243, 84.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.320 - 1.400
R-factor	0.1854
Rwork	0.184
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7mse
RMSD bond length	0.009
RMSD bond angle	1.088
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18_3845: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.320	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.040	0.690
Rmeas	0.042	0.722
Rpim	0.012	0.208
Number of reflections	61915	6095
<I/σ(I)>	31.44
Completeness [%]	100.0	100
Redundancy	12.7	11.8
CC(1/2)	1.000	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM Tris pH 8.0 2.25 M Ammonium sulfate